美国哥伦比亚大学2018年招聘博士后职位（电子显微镜学）

Postdoctoral Research Scientist

Columbia University Department of Surgery

Position Summary: The laboratory of Dr. Alex R. B. Thomsen is looking to recruit a Postdoctoral Research Scientist candidate for our newly initiated research program centered around compartmentalized G protein signaling by internalized G protein-coupled receptors (GPCRs) in the Department of Surgery, Columbia University Medical Center. The main objective of the research program is to understand the mechanistic basis behind compartmentalized GPCR signaling at a near-atomic level, and to study its direct implications on a variety of physiological and pathophysiological conditions including cancer metastasis, nociception, and salt/water homeostasis.

The primary project of the successful candidate will be to investigate the mechanism and contribution of compartmentalized chemokine receptor signaling to cell migration and metastasis of melanoma cells. The project will involve work with primary metastatic tissue isolated from patients as well as melanoma cell lines where an in-depth mechanism of cell migration is sought through usage of cryo-electron tomography and proteomics approaches. The insights learned from this project will be used to design future drug candidates to combat metastatic melanoma.

In addition, it is also expected that the applicant will participate in related secondary projects and collaborative projects within and/or outside our laboratory.

Requirements: We are looking for candidates with a Ph.D. and/or M.D. degree and experience in electron microscopy, proteomics, and/or cell biology. Prior knowledge of GPCR signal transduction is preferred but not essential. We are looking for motivated and creative individuals with a commitment to academic research who can work independently as well as in team-based projects. Good oral and written communication skills are musts.

Minimum Qualifications: PhD and/or MD in Biomedical Research required.

Preferred Qualifications: Electron Microscopy: Grid preparation, cryo-EM, cryo-electron tomography, negative stain EM, data processing.

Proteomics: Mass spectroscopy, APEX2, data processing.

Cellular and Molecular Biology: Cell culture, molecular cloning, cell migration assays, cell-based signaling assays, confocal and live cell fluorescence microscopy, genetic manipulation of cell lines by CRISPR and RNAi, etc.